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This study aimed to evaluate the phytochemical components, antimicrobial activity, and antioxidant activity of 80% ethanol extract of Scorzonera tomentosa, an endemic species. The chemical constituents of the ethanolic extract of S. tomentosa was primarily characterized by gas-chromatography-mass spectrometry analysis (GC-MS), ten components were identified. The major component was found as 2-pentanamine (35.68%). When the antioxidant capacity of S. tomentosa was examined, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities were determined to be quite high compared to the reference drug (IC50 values; DPPH: 517.0 ± 1.86 µg mL-1; ABTS: 244.8 ± 0.94 µg mL-1; reference drug: 1.313 ± 0.24 µg mL-1). In addition, according to total phenol content and total flavonoid content analyses, it was determined that the plant is richer in flavonoids. The antimicrobial activity of this species is not at an effective level. More extensive studies with S. tomentosa may allow the plant to be used as a natural antioxidant.