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This study aims to analyze whether the high-fat diet decreases serum TNF-α and breast tumor area on benzopyrene induced mice (Mus musculus). This study was a true experimental with the randomized posttest-only control group design using 36 female mice (Mus musculus), 3-4 months age, 25 ± 5 grams. Mice were induced with benzopyrene (BZP) subcutaneously with a dose of 0.3mg/20gBB/day for 14 days in the right breast area, then randomly divided into 6 groups, K1 (negative control group, given standard feed), K2 (positive control group, standard feed), K3 (high-fat diet with a ratio of 60% protein, 0% carbohydrate, 30% fat, 10% fiber), K4 (high-fat diet with a ratio of 45% protein, 0% carbohydrate, 45% fat, 10% fiber), K5 (high-fat diet with a ratio of 30% protein, 0% carbohydrate, 60% fat, 10% fiber) and K6 (high-fat diet on day 15 with a ratio of 15% protein, 0% carbohydrate, 75% fat, 10% fiber). The high-fat diet was administered for 28 days. The mean of tumor area delta at K1 (0.00 ± 0.00) mm2, K2 (3.52 ± 1.98) mm2, K3 (27.18 ± 21.23) mm2, K4 (13.19 ± 9.93) mm2, K5 (8.80 ± 1.72) mm2, K6 (10.81 ± 6.55) mm2, and (p=0.001). The mean of TNF-α levels at K1 (56.32 ± 8.25) ng/mL, K2 (65.99 ± 2.82) ng/mL, K3 (70.43 ± 4.61) ng/mL, K4 (58.05 ± 5.80) ng/mL, K5 (54.91 ± 3.27) ng/mL, K6 (59.67 ± 3.63) ng/mL and (P = 0.000). A high-fat diet lowers TNF-α levels and reduces the area of BZP-induced breast tumors. The lowest TNF-α levels and the lowest breast tumor area were found in groups with a fat: protein ratio = 60:30.