作者
Ni Huang, Paola Pérez, Takafumi Kato, Yu Mikami, Kenichi Okuda, Rodney C Gilmore, Cecilia Domínguez Conde, Billel Gasmi, Sydney Stein, Margaret Beach, Eileen Pelayo, Jose O Maldonado, Bernard A Lafont, Shyh-Ing Jang, Nadia Nasir, Ricardo J Padilla, Valerie A Murrah, Robert Maile, William Lovell, Shannon M Wallet, Natalie M Bowman, Suzanne L Meinig, Matthew C Wolfgang, Saibyasachi N Choudhury, Mark Novotny, Brian D Aevermann, Richard H Scheuermann, Gabrielle Cannon, Carlton W Anderson, Rhianna E Lee, Julie T Marchesan, Mandy Bush, Marcelo Freire, Adam J Kimple, Daniel L Herr, Joseph Rabin, Alison Grazioli, Sanchita Das, Benjamin N French, Thomas Pranzatelli, John A Chiorini, David E Kleiner, Stefania Pittaluga, Stephen M Hewitt, Peter D Burbelo, Daniel Chertow, HCA Oral and Craniofacial Biological Network http://orcid. org/0000-0003-3342-665X Sequeira Inês 36 http://orcid. org/0000-0002-6294-6366 A. Teichmann Sarah 1 26 J. Kimple Adam 3 20, Karen Frank, Janice Lee, Richard C Boucher, Sarah A Teichmann, Blake M Warner, Kevin M Byrd
发表日期
2021/5
期刊
Nature medicine
卷号
27
期号
5
页码范围
892-903
出版商
Nature Publishing Group US
简介
Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema and macules—the involvement of the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated and analyzed two single-cell RNA sequencing datasets of the human minor salivary glands and gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated cell normalization and annotation, we classified 34 unique cell subpopulations between glands and gingiva. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral entry factors such as ACE2 and TMPRSS members were broadly enriched in epithelial cells of the glands and oral mucosae. Using orthogonal RNA and protein expression assessments, we confirmed SARS-CoV-2 infection in the glands and mucosae. Saliva from SARS-CoV-2-infected individuals harbored …
学术搜索中的文章
N Huang, P Pérez, T Kato, Y Mikami, K Okuda… - Nature medicine, 2021