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In the present study, silver nanoparticles were synthesized by a simple, eco-friendly, cheap, non-toxic and sustainable method. The formation of the AgNPs was confirmed by UV-Vis spectroscopy in which the maximum absorption was observed at 421nm. The synthesized AgNPs were characterized using FTIR, XRD and HRTEM. The FTIR spectroscopy confirms the interactions between the phytochemical components of the extract and AgNPs in which the possible bio-molecules responsible for the reduction were identified.

Highly crystalline FCC structured AgNPs were confirmed from the peaks obtained in the XRD analysis. HRTEM showed dispersed spherical AgNPs with an average diameter of 20nm. Remarkably, the synthesized AgNPs exhibit superior antibacterial activity against K. Pneumonias and P. Aeruginosa gram-negative bacteria. The protein leakage from the AgNPs K. Pneumonias and P. Aeruginosa treated cells reveals the damage of cell membrane by the formation of pits like structure which may make cells inviable. The stressing of the cell structure and disruption of the cell wall were also observed using SEM analysis. Our results show that the synthesized AgNPs can be a superior candidate for effective bactericidal activity and also can be applied various the biomedical applications.