作者
Edward L Huttlin, Raphael J Bruckner, Jose Navarrete-Perea, Joe R Cannon, Kurt Baltier, Fana Gebreab, Melanie P Gygi, Alexandra Thornock, Gabriela Zarraga, Stanley Tam, John Szpyt, Brandon M Gassaway, Alexandra Panov, Hannah Parzen, Sipei Fu, Arvene Golbazi, Eila Maenpaa, Keegan Stricker, Sanjukta Guha Thakurta, Tian Zhang, Ramin Rad, Joshua Pan, David P Nusinow, Joao A Paulo, Devin K Schweppe, Laura Pontano Vaites, J Wade Harper, Steven P Gygi
发表日期
2021/5/27
期刊
Cell
卷号
184
期号
11
页码范围
3022-3040. e28
出版商
Elsevier
简介
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins—half the proteome—in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 cells. These networks model the interactome whose structure encodes protein function, localization, and complex membership. Comparison across cell lines validates thousands of interactions and reveals extensive customization. Whereas shared interactions reside in core complexes and involve essential proteins, cell-specific interactions link these complexes, "rewiring" subnetworks within each cell's interactome. Interactions …
学术搜索中的文章
EL Huttlin, RJ Bruckner, J Navarrete-Perea, JR Cannon… - Cell, 2021