作者
S Lavilla, JJ Gonzalez-Lopez, M Sabate, A Garcia-Fernandez, MN Larrosa, RM Bartolome, A Carattoli, G Prats
发表日期
2008/2/1
期刊
Journal of antimicrobial chemotherapy
卷号
61
期号
2
页码范围
291-295
出版商
Oxford University Press
简介
Objectives
To evaluate the presence of qnr genes among enterobacterial isolates carrying extended-spectrum β-lactamases (ESBLs) in Barcelona, Spain.
Methods
Screening for the qnrA, qnrB and qnrS genes was carried out by PCR amplification with specific primers in 305 non-duplicate, clinically relevant ESBL-producing enterobacterial isolates obtained from February 2003 to August 2004. ESBLs from all qnr-positive isolates were characterized by isoelectric focusing, PCR amplification and DNA sequencing. Plasmid analysis was performed by S1 digestion and hybridization with specific probes for the qnr and bla genes. Plasmids containing qnr genes were transferred by conjugation or transformation. The genetic environment of qnrA1 in selected isolates was characterized by cloning experiments.
Results
Fifteen isolates, each from a different …
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