作者
Somin Lee, Habin Kang, Dohyun Park, James Yu, Seung Kwon Koh, Duck Cho, Da‐Hyun Kim, Kyung‐Sun Kang, Noo Li Jeon
发表日期
2021/1/14
期刊
Advanced Biology
页码范围
2000195
简介
The lymphatic vessel (LV) plays an important role in cancer biology as a major route for tumor metastasis. Whereas, recent oncoimmunological approaches have focused its role in immune surveillance. In response to the emerging topics of lymphatic vascular biology, physiologically relevant human cell‐based in vitro model is in high demand. This study introduces a 3D in vitro model of human LV within tumor immune microenvironment (TIME) using an injection‐molded plastic array culture platform (Lymph‐IMPACT). Through spontaneous capillary flow‐driven patterning of 3D cellular hydrogel and optimized cellular composition, the platform enables robust and reproducible formation of self‐organized LV in vitro. This co‐culture model recapitulates cancer cell type‐dependent morphogenesis of LV in vitro. Moreover, the robustness of the model enables high‐content analysis on the effect of anti‐VEGFR3 drug …
学术搜索中的文章
S Lee, H Kang, D Park, J Yu, SK Koh, D Cho, DH Kim… - Advanced Biology, 2021