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To determine whether the transfer of blastocysts on day 5, developed in sequential culture media, resulted in an increase in implantation rate compared with embryos transferred on day 3.

Comparative study of embryo culture regimes.

Private practice assisted reproductive technology center. Patient(s): Twenty-three patients undergoing routine IVF cycles. Intervention(s): Culture of embryos to day 3 in either standard culture conditions or a serum-free chemically defined medium. One hundred one embryos were subsequently cultured from day 3 to day 5 in a second serum-free medium specifically designed to support development of the blastocyst. Main Outcome Measure(s): Embryo cell number and quality on day 3. Blastocyst development on day 5. Implantation rate (determined by fetal heart) and ongoing pregnancy rate (PR). Result(s): Implantation rates for embryos transferred at the …