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Results: VitD did not significantly change the expression of MMP-9, MMP-2, TIMP-1 and TIMP-2 at mRNA level at various incubation times and concentrations. The expression of OPG at mRNA was significantly increased in the presence of TNF-a and this effect became more pronounced when cells were preincubated with Vit D at concentration 10− 9 M. Preincubation with p38 MAP kinase inhibitor totally abolished this result.

When cells activated with TNF-a, preincubation with Vit D resulted in a significant decrease in MMP-9 expression at 10− 10 and 10− 11 M and increase in TIMP-2 expression while it didn’t affect the expression of MMP-2 and TIMP-1. Gelatin zymography revealed that Vit D decrease the active MMP-2 dose-dependently.

Conclusions: Vit D at physiologically achievable concentrations reduces the expression of MMP-9 and induces the expression of TIMP-2 at mRNA level implying a possible …