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Results: After 11 days of culture, incubation of PBMCs with dexamethasone for the last 48-h resulted in 12-fold increase in GILZ-mRNA levels. When PBMCs were pre-incubated with 1, 25 (OH) 2 D 3, dexamethasone (last 48-h) induced a significant down-regulation of GILZ mRNA expression. When PBMCs were pre-treated with 1, 25 (OH) 2 D 3 for 11 days, dexamethasone (last 48-h) caused a significant down-regulation of GRα mRNA expression, confirmed at protein level. Treatment of PBMCs with 1, 25 (OH) 2 D 3, led to significant decrease in FKBP5 and NCoR1 mRNA levels. IF staining showed that vitamin D causes a GR translocation from nucleus to cytoplasm.

Conclusion: 1, 25 (OH) 2 D 3 decreased the in vitro glucocorticoid sensitivity in PBMCs isolated from healthy women. This effect is, at least in part, attributed to the reduction of GRα expression due to a mechanism that did not appear to implicate NCoR1 …