作者
Peter Willeit, Anna Zampetaki, Katarzyna Dudek, Dorothee Kaudewitz, Alice King, Nicholas S Kirkby, Roxanne Crosby-Nwaobi, Marianna Prokopi, Ignat Drozdov, Sarah R Langley, Sobha Sivaprasad, Hugh S Markus, Jane A Mitchell, Timothy D Warner, Stefan Kiechl, Manuel Mayr
发表日期
2013/2/15
期刊
Circulation research
卷号
112
期号
4
页码范围
595-600
出版商
Lippincott Williams & Wilkins
简介
Rationale:
MicroRNA (miRNA) biomarkers are attracting considerable interest. Effects of medication, however, have not been investigated thus far.
Objective:
To analyze changes in plasma miRNAs in response to antiplatelet therapy.
Methods and Results:
Profiling for 377 miRNAs was performed in platelets, platelet microparticles, platelet-rich plasma, platelet-poor plasma, and serum. Platelet-rich plasma showed markedly higher levels of miRNAs than serum and platelet-poor plasma. Few abundant platelet miRNAs, such as miR-24, miR-197, miR-191, and miR-223, were also increased in serum compared with platelet-poor plasma. In contrast, antiplatelet therapy significantly reduced miRNA levels. Using custom-made quantitative real-time polymerase chain reaction plates, 92 miRNAs were assessed in a dose-escalation study in healthy volunteers at 4 different time points: at baseline without therapy, at 1 week …
学术搜索中的文章
P Willeit, A Zampetaki, K Dudek, D Kaudewitz, A King… - Circulation research, 2013
