查看文章

The adsorption of cellulase enzymes onto lignin is shown to be non‐productive and therefore reduces enzymatic hydrolysis of liquid hot water pretreated cellulose. Among the enzyme components of Trichoderma reesei cellulase cocktail, β‐glucosidase showed the strongest adsorption onto lignin. Only 2–18% of the initial β‐glucosidase activity remained in the supernatant while 50–60% of cellobiohydrolase and endoglucanase activities were recovered after incubation with lignin. By increasing the pH to 5.5 and adding NaCl to a 200 mM, the free enzymes in the supernatant were increased but hydrolysis was not enhanced since optimal pH for enzymatic hydrolysis is at 4.8. Electrostatic interactions contributed to enzyme adsorption and their effect was most pronounced for T. reesei β‐glucosidase which had high molecular weights (78–94 kDa) and high isoelectric points (pI 5.7–6.4). Since the enzyme …