查看文章

Human hepatocellular carcinoma (HCC) is one of the most common causes of death in the world. Targeted drug delivery to cells, tissues or specialized receptor cells is an advanced technology in treatment of HCC. The purpose of this study was to investigate the cytotoxicity properties of DTX nanoparticles. In this research nanoparticles were prepared through radical polymerization. HepG2 cells were seeded in 96-well plates at a density of 10,000 viable cells per well. Then 0.01-0.50 µg/ml of the nanoparticle and the free drug was added the day after seeding. Afterwards, the number of viable cells was counted and the activity of mitochondrial dehydrogenase enzymes of the cells was detected in 24, 48 and 72 hrs using MTT assay. The results of MTT assay showed strong and dose-dependent inhibition of HepG2 carcinoma cell growth of the nanoparticle compared with DTX. Inhibitory concentrations (IC50) that was obtained for nanoparticles and free drug incubation times of 24, 48 and 72 hours were 1.02±0.68, 0.39±0.86, 0.20±0.93 and 10.39±1.34, 8.87±0.97, 5.99±0.76 µg/ml, respectively. The results showed higher cytotoxity effect of nanoparticles in comparison with free drug against HepG2 cell lines in all mentioned incubation times. Hence, thiolated-chitosan nanoparticles could be a potentially useful delivery system for docetaxel as an anticancer agent in treatment of liver cancer.