作者
Rafael Yaari, Chen Noy-Malka, Gertrud Wiedemann, Nitzan Auerbach Gershovitz, Ralf Reski, Aviva Katz, Nir Ohad
发表日期
2015/7
期刊
Plant molecular biology
卷号
88
页码范围
387-400
出版商
Springer Netherlands
简介
DNA methylation has a crucial role in plant development regulating gene expression and silencing of transposable elements. Maintenance DNA methylation in plants occurs at symmetrical mCG and mCHG contexts (m = methylated) and is maintained by DNA METHYLTRANSFERASE 1 (MET1) and CHROMOMETHYLASE (CMT) DNA methyltransferase protein families, respectively. While angiosperm genomes encode for several members of MET1 and CMT families, the moss Physcomitrella patens, serving as a model for early divergent land plants, carries a single member of each family. To determine the function of P. patens PpMET we generated ΔPpmet deletion mutant which lost mCG and unexpectedly mCCG methylation at loci tested. In order to evaluate the extent of mCCG methylation by MET1, we reexamined the Arabidopsis thaliana Atmet1 mutant methylome and found a similar pattern of …
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