作者
Takashi Ando, Kyosuke Hatsushika, Masanori Wako, Tetsuro Ohba, Kensuke Koyama, Yuko Ohnuma, Ryohei Katoh, Hideoki Ogawa, Ko Okumura, Jian Luo, Tony Wyss-Coray, Atsuhito Nakao
发表日期
2007/10/1
期刊
Journal of Allergy and Clinical Immunology
卷号
120
期号
4
页码范围
916-923
出版商
Mosby
简介
BACKGROUND
Epidemiologic studies suggest that TGF-β in breast milk provides protection against allergic disease during infancy. However, it is unclear whether orally administered TGF-β, such as TGF-β in human milk, retains and exerts its activity in the intestinal mucosa and can affect immune response (tolerance) to dietary antigens.
OBJECTIVE
We sought to determine whether orally administered TGF-β is biologically active in intestinal mucosa and affects oral tolerance.
METHODS
Activity of orally administered TGF-β in the intestinal mucosa was evaluated by means of in vivo imaging with transgenic mice expressing a Smad-responsive reporter construct (SBE-luc mice), by means of immunohistochemical staining with anti-phosphorylated Smad2 antibody, and by means of real-time RT-PCR analysis of TGF-β and Smad7 mRNA expression. The effects of orally administered TGF-β on oral tolerance induction …
引用总数
学术搜索中的文章
T Ando, K Hatsushika, M Wako, T Ohba, K Koyama… - Journal of Allergy and Clinical Immunology, 2007