查看文章

Purpose: Primary vitreoretinal lymphoma diagnostics are greatly limited by cellular preservation of morphology, DNA and RNA integrity. Molecular analysis and staining properties of liquid biopsies are affected by the type of fixative and fixation protocols. This study aims to determine the optimal fixative and fixation method to obtain efficiently high-quality RNA and DNA for further downstream molecular analyses and cellular markers staining for flow cytometry (FACS).

Methods: Six different common fixative solutions (RNAlater™, HOLOGIC-PreservCyt Solution, PAXgene Tissue Container, Shandon™ Cytospin™ Collection Fluid, Allprotect Tissue Reagent and HOPE® I solution) were included in the comparison. Fresh unfixed Germinal center B-cell-like DLBCL cell line Pfeiffer (ATCC CRL2632) as controls. The equivalent amount of Pfeiffer cells were fixed immediately after harvesting and stored at− 4 C for seven days …