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Purpose: To determine the role of single cells molecular analysis from remnant cytology samples for vitreoretinal lymphoma (VRL) diagnosis. Vitreoretinal lymphoma is often difficult to diagnosis due to the paucicellular yield of fragile VRL tumour cells. Single cell molecular analysis may provide further genetic data to aid clinical diagnosis.

Methods: This study was performed in compliance with the Declaration of Helsinki with informed consent and SingHealth institutional review board approval. A total of 4 patient remnant samples of aqueous/vitreous fixed in PreservCyt® was obtained from the Singapore General Hospital Department of Anatomical Pathology and Cytology after the diagnosis was confirmed. IgH rearrangement were performed as a part of clinical diagnosis. Two samples were confirmed vitreoretinal large B cell lymphomas and 2 were inflammatory controls. Single B (CD20+/CD3-/CD19+) cells were …