作者
Wei Jian Tan, Mona Meng Wang, Paola Ricciardi Castagnoli, Tiffany Tang, Anita Sook Yee Chan, Tong Seng Lim
发表日期
2021/7/1
期刊
Ophthalmology
卷号
128
期号
7
页码范围
1079-1090
出版商
Elsevier
简介
Purpose
To test whether analyzing DEPArray (Menarini Silicon Biosystems) isolated single B cells from the vitreous fluid can reveal crucial genomic and clinicopathological features to distinguish patients with vitreoretinal lymphoma (VRL) from those with chronic inflammation using immunoglobulin heavy chain (IGH), disease biomarker myeloid differentiation primary response 88 (MYD88)L265P mutation, and copy number profiling.
Design
A single-center, retrospective study.
Participants
Remnant vitreous biopsies from 7 patients with VRL and 4 patients with chronic inflammation were acquired for molecular analysis.
Methods
Vitreous fluid samples were prefixed in PreservCyt (Hologic) and underwent cytologic analysis and immunohistochemistry examination. Single cells were isolated using the DEPArray NxT system, followed by downstream genomic analysis.
Main Outcome Measures
The frequencies of the …
学术搜索中的文章
WJ Tan, MM Wang, PR Castagnoli, T Tang, ASY Chan… - Ophthalmology, 2021