作者
Mona Meng Wang, Wei Jian Tan, Tong Seng Lim, Anita Sook Yee Chan
发表日期
2021/4/3
期刊
Ocular Immunology and Inflammation
卷号
29
期号
3
页码范围
430-439
出版商
Taylor & Francis
简介
Purpose: Vitreoretinal lymphoma (VRL) is a potentially fatal intraocular malignancy. Diagnosis is hampered by poor preservation of morphology and DNA/RNA integrity, which precludes adjunctive molecular analysis. We aimed to determine the optimum fixative protocol for VRL biopsies that permits cytology, IHC/flow cytometry and molecular analyses.
Methods: Six fixatives were compared on cultured Pfeiffer cells used as a cellular model. Cells were fixed and evaluated on cellular morphology, antibody staining, DNA/RNA amount and integrity. VRL clinical cases were used as validation and proof-of-concept.
Results: PreservCyt was the best fixative for preserving cellular morphology and high-quality RNA/DNA from vitreous fluid biopsies. Cells from clinical VRL cases fixed with PreservCyt showed adequate cellular morphology and IHC positivity. Sufficient DNA was obtained for IgH clonality and MYD88 mutation …
学术搜索中的文章
MM Wang, WJ Tan, TS Lim, ASY Chan - Ocular Immunology and Inflammation, 2021