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Purpose: The ability to sort and recover rare target immune cells from small volume of liquid biopsies such as ocular fluids poses unique challenges that impede diagnostic accuracy. Heterogeneous cell populations with non-target immunoreactive cells in paucicellular liquid biopsies complicate conventional bulk-cell analysis, and could lead to disease misdiagnosis. This study tested the hypothesis that comprehensive molecular and genetic characterization of pathogenic immune cells at single cell resolution enables the precision diagnosis of ocular malignancies.

Methods: Study was performed in compliance with the Declaration of Helsinki with informed consent and Singhealth institutional review board approval. We provided proof-of-concept that DEPArray™ technology enabled automated isolation and recovery of rare B cells or T cells from small volumes of ocular aqueous/vitreous fluids from vitreoretinal …