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Staphylococcus epidermidis, Escherichia coli and Candida albicans were tested. Both essential oils possessed significant antimicrobial effects against all microorganisms tested. The MICs of clove oil ranged from 0.062% to 0.500%(v/v), while the MICs of rosemary oil ranged from 0.125% to 1.000%(v/v). The antimicrobial activity of combinations of the two essential oils indicated their additive, synergistic or antagonistic effects against individual microorganism tests. The time-kill curves of clove and rosemary essential oils towards three strains showed clearly bactericidal and fungicidal processes of 1/2× MIC, MIC, MBC and 2× MIC. Copyright© 2007 John Wiley & Sons, Ltd.