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In this study, the influence of supercritical extraction parameter on the oil extraction of Nutmeg (Myristica fragrans) was investigated, and then the extracted oil was tested for its cytotoxic and anti-angiogenic activity. Supercritical extraction was carried out using the operation pressures 20.7, 27.6, 34.5 and 41.4 MPa, and temperature was varied between 40 and 50 °C. The CO₂ flow rate was between 1-3 ml/min using matrix particle size ≤ 1 mm and during extraction time of 90 min. The extracted oils were tested for anti-angiogenic activity using a 3 dimensional ex-vivo isolated rat aorta tissue. MTT assay was used as in vitro study to investigate the cytotoxic properties of the extracted oil samples on two types of human tumour cell lines, colon cancer cell HCT-116 and breast cancer cell MCF7. The study reveal that the extraction yield depends on pressure and temperature, and there is a significant difference between …