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This paper focused on thermal conductivity and viscosity of deionised water and ethylene glycol-based nanofluids at three different temperatures (6 C, 25 C and 40 C). For the preparation of nanofluids, a two-step method, comprised of homogenisation and sonication, was used on a mixture of MWCNT-OH, PVP and the base fluid. The results revealed that thermal conductivity was enhanced by about 8.86% for 0.8 wt% deionised water-based MWCNT-OH nanofluid, and by 5.37% for 0.2 wt% ethylene glycol-based MWCNT-OH nanofluid. Meanwhile, in viscosity test, the highest temperature of 40 C exhibited lowest viscosity. This phenomenon happened only with ethylene glycol-based nanofluid, whilst the data on the viscosity of deionised water-based nanofluid was inconsistent at certain nanofluid concentrations. In conclusion, addition of MWCNT-OH into base fluid enhanced base fluid performance, giving it the potential to be used in cooling system applications.