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The aim of this work is to study the survival of genome and infectious units of adenovirus type 40, rotavirus Wa strain and Hepatitis A virus (HAV) HM 175 adapted strain in Egyptian ground water at different temperatures and their relationships with the persistence of bacterial indicators and pathogenic bacteria. Also, study the effect of limestone on the same viruses and bacteria in ground water. The genome of adenovirus was the more stable and persistent followed by rotavirus Wa strain and finally HAV HM 175 adapted strain. There were gradual decreases in the genome of the three tested viruses parallel with the time. After 12 weeks, adenovirus type 40 genome copies reductions were 0.05 log, 0.5 log and 1.5 log at 4 C, 22 C and 35 C