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Increasing the xenobiotic concentration by anthropogenic activity on the planet has led to toxic human health and reduced the biodiversity of the natural ecosystem. Enzymatic bioremediation can provide real benefits to the environment, avoiding the conditions that are required for whole-cell applications, especially in extreme environments. Bacterial consortia are a better alternative for the degradation of diverse and complex xenobiotic compounds. Likewise, the use of an enzyme mixture is probably a more suitable tool for use against xenobiotic contamination in the environment because specific enzymes for recalcitrant and toxic compounds can be applied together. Furthermore, enzymatic effectiveness can be improved in vitro also using molecular tools, such as DNA engineering, to generate super bioremediators, which can present advantages in the field.