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Drought is one of the major constraints limiting crop productivity in African Sahel. The aim of this study was to select mutant sesame (Sesamum indicum L.) lines with improved levels of drought resistance. Twenty-one M4-M5 sesame lines of unknown drought tolerance, and their three parental sources with well-known and contrasting drought tolerance levels were evaluated at the vegetative stage in a factorial pot experiment, using a completely randomized design with three replicates. After 2 weeks of growth, water was withheld for 16 days as drought stress treatment. Chlorophyll a fluorescence data, as well as stomatal conductance and flag leaf temperature were recorded during the stress period. Recorded chlorophyll a fluorescence transients were analyzed by the JIP-test to translate stress-induced damage in these transients to changes in biophysical parameters allowing quantification of the energy flow …