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The objective was to determine the frequency of occurrence of alloantibodies among pregnant women in Iran.

This was a prospective cross‐sectional study, which was carried out in the immunohematology reference laboratory of the Iranian Blood Transfusion Organization in Tehran, Iran, in 2008 to 2015. Screening and identification of red blood cell (RBC) alloantibodies was done on the sera of 7340 pregnant females using the standard tube method and gel column agglutination technique.

Alloantibodies were identified in the serum of 332 of the 7340 (4.5%) pregnant women. A total of 410 antibodies were detected in 332 positive maternal serum samples with no previous history of blood transfusion. Anti‐D was the most common antibody accounting for 70.5% of all the antibodies formed in D– women. The incidence of specific alloimmunization other …