作者
Anil Purohit, Paari Dominic Swaminathan, Biyi Chen, Niels Voigt, Olha M Koval, Elizabeth D Luczak, Zhan Gao, Na Li, Xiaoqun Guan, Beixin J He, Ramzi N El-Accaoui, Jinying Yang, William Kutschke, Madhu V Singh, Robert M Weiss, Xander H Wehrens, Long-Sheng Song, Dobromir Dobrev, Mark E Anderson
发表日期
2011/11/22
来源
Circulation
卷号
124
期号
suppl_21
页码范围
A14037-A14037
出版商
Lippincott Williams & Wilkins
简介
Introduction: Atrial Fibrillation (AF) is favored by Angiotensin II (Ang II) and reactive oxygen species (ROS), but the mechanism is unknown. We hypothesized that Ang II causes oxidative activation of calcium-calmodulin kinase II (CaMKII), leading to increased SR calcium (Ca2+) release by ryanodine receptor (RyR2) phosphorylation and thus promoting AF.
Methods: We established a mouse model of AF by implanting subcutaneous Ang II eluting osmotic pumps (2000 ng/kg/min) for three weeks. After three weeks, in vivo burst pacing was performed in the right atrium of anesthetized mice to induce AF. We used antibodies against oxidized and total CaMKII, for immunofluorescence imaging and immunoblotting of mouse hearts or human right atrial tissue from patients with and without AF.
Results: Atrial tissue from patients with AF and Ang II treated mice showed higher oxidized CaMKII signal as compared to …
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A Purohit, P Dominic Swaminathan, B Chen, N Voigt… - 2011