作者
Mihaela Crisan, Louis Casteilla, Lorenz Lehr, Mamen Carmona, Ariane Paoloni-Giacobino, Solomon Yap, Bin Sun, Bertrand Léger, Alison Logar, Luc Pénicaud, Patrick Schrauwen, David Cameron-Smith, Aaron Paul Russell, Bruno Péault, Jean-Paul Giacobino
发表日期
2008/9/1
期刊
Stem cells
卷号
26
期号
9
页码范围
2425-2433
出版商
Oxford University Press
简介
Brown adipose tissue uncoupling protein-1 (UCP1) plays a major role in the control of energy balance in rodents. It has long been thought, however, that there is no physiologically relevant UCP1 expression in adult humans. In this study we show, using an original approach consisting of sorting cells from various tissues and differentiating them in an adipogenic medium, that a stationary population of skeletal muscle cells expressing the CD34 surface protein can differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression and uncoupled respiration. These cells can be expanded in culture, and their UCP1 mRNA expression is strongly increased by cell-permeating cAMP derivatives and a peroxisome-proliferator-activated receptor-γ (PPARγ) agonist. Furthermore, UCP1 mRNA was detected in the skeletal muscle of adult humans, and its expression was increased in vivo by PPARγ …
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M Crisan, L Casteilla, L Lehr, M Carmona… - Stem cells, 2008