作者
Claudia I Chapuy, Maria D Aguad, Rachel T Nicholson, James P AuBuchon, Claudia S Cohn, Meghan Delaney, Mark K Fung, Meredith Unger, Parul Doshi, Michael F Murphy, Larry J Dumont, Richard M Kaufman, DARA‐DTT Study Group* for the BEST Collaborative
发表日期
2016/12
期刊
Transfusion
卷号
56
期号
12
页码范围
2964-2972
简介
BACKGROUND
Daratumumab (DARA) consistently interferes with routine blood bank serologic testing by directly binding to CD38 expressed on reagent red blood cells (RBCs). Treating RBCs with dithiothreitol (DTT) eliminates the DARA interference. We conducted an international, multicenter, blinded study aimed at validating the DTT method for use by blood bank laboratories worldwide.
STUDY DESIGN AND METHODS
Paired plasma sample unknowns were sent to 25 participating blood bank laboratories. Sample 1 was spiked with DARA only (10 µg/mL), and Sample 2 with DARA plus a clinically significant RBC antibody (anti‐D [n = 6], anti‐Fya [n = 9], or anti‐s [n = 10]). Sites were instructed to perform an antibody screen with and without DTT‐treated RBCs and to use a DTT‐treated RBC panel for antibody identification. Qualitative data about the DTT method were collected by online survey. The primary …
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