作者
Wei Zhang, Yuan Shi, Yanzuo Chen, Shuangyin Yu, Junguo Hao, Jieqi Luo, Xianyi Sha, Xiaoling Fang
发表日期
2010/8/1
期刊
European journal of pharmaceutics and biopharmaceutics
卷号
75
期号
3
页码范围
341-353
出版商
Elsevier
简介
The aim of this work was to demonstrate the advantage of using paclitaxel (PTX)-loaded Pluronic P123/F127 mixed micelles (PF-PTX) against non-small cell lung cancer (NSCLC) compared to Taxol. Modulation of multidrug resistance (MDR) by Pluronic mixed micelles was evaluated in lung resistance protein (LRP)-overexpressing human lung adenocarcinoma A-549 cell line. Influence of PF-PTX on in vitro cytotoxicity was determined by MTT assay, while cellular apoptosis was detected by cell nuclei staining and Annexin V-FITC apoptosis detection kit. Cell cycle arrest was also confirmed by flow cytometry. Additionally, in vivo fate and antitumor efficacy of PF-PTX were extensively evaluated in comparison with Taxol. It was demonstrated that PF-PTX had superior anti-proliferation activity against A-549 cells compared with Taxol as measured by IC50. The enhanced anti-cancer efficacy of PF-PTX was associated …
引用总数
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W Zhang, Y Shi, Y Chen, S Yu, J Hao, J Luo, X Sha… - European journal of pharmaceutics and …, 2010