作者
Xin Wu, Baoyue Ding, Jing Gao, Huanyun Wang, Wei Fan, Xiang Wang, Wei Zhang, Xiaoyu Wang, Lihua Ye, Min Zhang, Xueying Ding, Jiyong Liu, Quangang Zhu, Shen Gao
发表日期
2011/8/19
期刊
International journal of nanomedicine
页码范围
1747-1756
出版商
Taylor & Francis
简介
Background
miR-15a and miR-16-1 have been identified as tumor suppressor genes in prostate cancer, but their safe and effective delivery to target cells is key to the successful use of this therapeutic strategy. RNA aptamer A10 has been used as a ligand, targeting prostate cancer cells that express prostate-specific membrane antigen (PSMA). Compared with A10, the binding of the second-generation RNA aptamer, A10-3.2, to PSMA is more efficient.
Methods
A10-3.2 was investigated as a PSMA-targeting ligand in the design of a polyamidoamine (PAMAM)-based microRNA (miR-15a and miR-16-1) vector to prostate cancer cells. Using polyethyleneglycol (PEG) as a spacer, PAMAM was conjugated to aptamer (PAMAM-PEG-APT) and used as a vehicle for miRNA target delivery.
Results
Luciferase assays of pGL-3 expression against PC3 (PSMA−) and LNCaP (PSMA+) cells demonstrated that the transfection …
学术搜索中的文章
X Wu, B Ding, J Gao, H Wang, W Fan, X Wang… - International journal of nanomedicine, 2011