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Clustered regularly interspaced short palindromic repeat-CRISPR-associated protein (CRISPR-Cas) genome-editing systems, prokaryotic adaptive immune system, have been exploited and prepared a robust programmable method for efficient and accurate genome editing and gene targeting. This revolutionary technique can be used for engineered cell therapies, in vivo gene therapy, animal modeling, and cancer diagnosis and treatment. Recently, safe and efficient delivery is one of the greatest challenges in the clinical utilization of CRISPR/Cas9. Though using viral delivery strategies have many advantages for both in vitro and in vivo genome editing, their severe impediments like small insertion size, high carcinogenesis risk, and immune system stimulation, cause many problems for both laboratory and clinical applications. Consequently, there is a critical need for the development of novel non-viral delivery …