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Objective

Sperm lipids play a very important role in the integrity of membranes and determine many of their important physicochemical properties. However, data on the lipid profile of human sperm cells is currently very limited and mainly obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, in which resulting spectra are largely dominated by phosphatidylcholine (PC) and lyso-phosphatidylcholine signals. By MRM-profiling, diverse lipid classes can be profiled using a parent ion and class fragment information to obtain multiple reaction monitoring (MRM) methods. Thus, we aimed to characterize the lipid profile of sperm of fertile and subfertile men by MRM-profiling, which may help to identify possible lipids related to male fertility.

Design