作者
Andrew Donovan Young, Alan R Lemmon, Jeffrey H Skevington, Ximo Mengual, Gunilla Ståhls, Menno Reemer, Kurt Jordaens, Scott Kelso, Emily Moriarty Lemmon, Martin Hauser, Marc De Meyer, Bernhard Misof, Brian M Wiegmann
发表日期
2016/12
期刊
BMC evolutionary biology
卷号
16
页码范围
1-13
出版商
BioMed Central
简介
Background
Anchored hybrid enrichment is a form of next-generation sequencing that uses oligonucleotide probes to target conserved regions of the genome flanked by less conserved regions in order to acquire data useful for phylogenetic inference from a broad range of taxa. Once a probe kit is developed, anchored hybrid enrichment is superior to traditional PCR-based Sanger sequencing in terms of both the amount of genomic data that can be recovered and effective cost. Due to their incredibly diverse nature, importance as pollinators, and historical instability with regard to subfamilial and tribal classification, Syrphidae (flower flies or hoverflies) are an ideal candidate for anchored hybrid enrichment-based phylogenetics, especially since recent molecular phylogenies of the syrphids using only a few markers have resulted in highly unresolved topologies. Over 6200 syrphids are …
引用总数
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