作者
Elizabeth S Fernandes, Fiona A Russell, Domenico Spina, Jason J McDougall, Rabea Graepel, Clive Gentry, Amelia A Staniland, David M Mountford, Julie E Keeble, Marzia Malcangio, Stuart Bevan, Susan D Brain
发表日期
2011/3
期刊
Arthritis & Rheumatism
卷号
63
期号
3
页码范围
819-829
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
To investigate the involvement of transient receptor potential ankyrin 1 (TRPA1) in inflammatory hyperalgesia mediated by tumor necrosis factor α (TNFα) and joint inflammation.
Methods
Mechanical hyperalgesia was assessed in CD1 mice, mice lacking functional TRP vanilloid 1 (TRPV1−/−) or TRPA1 (TRPA1−/−), or respective wild‐type (WT) mice. An automated von Frey system was used, following unilateral intraplantar injection of TNFα or intraarticular injection of Freund's complete adjuvant (CFA). Knee swelling and histologic changes were determined in mice treated with intraarticular injections of CFA.
Results
TNFα induced cyclooxygenase‐independent bilateral mechanical hyperalgesia in CD1 mice. The selective TRPV1 receptor antagonist SB‐366791 had no effect on mechanical hyperalgesia when it was coinjected with TNFα, but intrathecally administered SB‐366791 attenuated bilateral …
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ES Fernandes, FA Russell, D Spina, JJ McDougall… - Arthritis & Rheumatism, 2011