作者
Marie Deprez, Laure-Emmanuelle Zaragosi, Marin Truchi, Christophe Becavin, Sandra Ruiz García, Marie-Jeanne Arguel, Magali Plaisant, Virginie Magnone, Kevin Lebrigand, Sophie Abelanet, Frédéric Brau, Agnès Paquet, Dana Pe’er, Charles-Hugo Marquette, Sylvie Leroy, Pascal Barbry
发表日期
2020/12/15
期刊
American journal of respiratory and critical care medicine
卷号
202
期号
12
页码范围
1636-1645
出版商
American Thoracic Society
简介
Rationale: The respiratory tract constitutes an elaborate line of defense that is based on a unique cellular ecosystem.
Objectives: We aimed to investigate cell population distributions and transcriptional changes along the airways by using single-cell RNA profiling.
Methods: We have explored the cellular heterogeneity of the human airway epithelium in 10 healthy living volunteers by single-cell RNA profiling. A total of 77,969 cells were collected at 35 distinct locations, from the nose to the 12th division of the airway tree.
Measurements and Main Results: The resulting atlas is composed of a high percentage of epithelial cells (89.1%) but also immune (6.2%) and stromal (4.7%) cells with distinct cellular proportions in different regions of the airways. It reveals differential gene expression between identical cell types (suprabasal, secretory, and multiciliated cells) from the nose (MUC4, PI3, SIX3) and tracheobronchial …
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M Deprez, LE Zaragosi, M Truchi, C Becavin… - American journal of respiratory and critical care …, 2020