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Seed extracts of Moringa oleifera were assayed for the evaluation of antimicrobial activity against bacterial (Pasturella multocida, Escherichia coli, Bacillus subtilis and Staphlocuccus aureus) and fungal (Fusarium solani and Rhizopus solani) strains. The crude, supernatant, residue and dialyzed samples inhibited the growth of all microbs to various extents. The zones of growth inhibition showed greater sensitivity against the bacterial strains as compared to the fungal strains. The extracts worked in dose dependent manner and resulted in crippled and distorted hyphae and apical branching in fungi. Minimum inhibitory concentrations (MIC) extracts revealed that Pasturella multocida and Bacillus subtilis were most sensitive strains. However, the activity of the extracts was antagonized by cations (Na+, K+, Mg2+ and Ca2+). Maximum activity was found between temperature 4-37 OC and pH 7.