作者
Janneke GF Hogervorst, Roger WL Godschalk, Piet A van den Brandt, Matty P Weijenberg, Bas AJ Verhage, Leonie Jonkers, Joy Goessens, Colinda CJM Simons, Joris R Vermeesch, Frederik J van Schooten, Leo J Schouten
发表日期
2014/12/1
来源
Cancer Epidemiology, Biomarkers & Prevention
卷号
23
期号
12
页码范围
2703-2712
出版商
American Association for Cancer Research
简介
Background: Nails contain genomic DNA that can be used for genetic analyses, which is attractive for large epidemiologic studies that have collected or are planning to collect nail clippings. Study participants will more readily participate in a study when asked to provide nail samples than when asked to provide a blood sample. In addition, nails are easy and cheap to obtain and store compared with other tissues.
Methods: We describe our findings on toenail DNA in terms of yield, quality, genotyping a limited set of SNPs with the Sequenom MassARRAY iPLEX platform and high-density genotyping with the Illumina HumanCytoSNP_FFPE-12 DNA array (>262,000 markers). We discuss our findings together with other studies on nail DNA and we compare nails and other frequently used tissue samples as DNA sources.
Results: Although nail DNA is considerably degraded, genotyping …
学术搜索中的文章
JGF Hogervorst, RWL Godschalk, PA van den Brandt… - Cancer Epidemiology, Biomarkers & Prevention, 2014