作者
Li Sun, Huaqing Cai, Weihong Xu, Yuanlei Hu, Yin Gao, Zhongping Lin
发表日期
2001/12
期刊
Plant Molecular Biology Reporter
卷号
19
页码范围
383-384
出版商
Springer-Verlag
简介
Ganoderma lucidum is a well-known and important medicinal mushroom, but its genetic modification has not been reported. We developed an efficient procedure for isolation and regeneration of protoplasts fromG. lucidum. To construct a vector for high-level expression of heterologous genes inG. lucidum, the 1.4-kb regulatory region of the glyceraldehyde-3-phosphate dehydrogenase gene (GPD) was isolated from the genomic DNA ofLentinus edodes, and theGPD promoter was fused to the β-glucuronidase (GUS) and bialaphos resistance (bar) genes. Using the resulting construct, p301-bG1, an efficient transformation system based on electroporation was established forG. lucidum. GUS expression was observed among transformants conferring bialaphos resistance, indicating that theL. edodes GPD promoter can be used for expression of exogenous genes inG. lucidum. We also studied green …
引用总数
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L Sun, H Cai, W Xu, Y Hu, Y Gao, Z Lin - Plant Molecular Biology Reporter, 2001