作者
Weihong Xu, Jennifer G Aparicio, Oscar M Aparicio, Simon Tavaré
发表日期
2006/12
期刊
BMC genomics
卷号
7
页码范围
1-16
出版商
BioMed Central
简介
Background
Eukaryotic replication origins exhibit different initiation efficiencies and activation times within S-phase. Although local chromatin structure and function influences origin activity, the exact mechanisms remain poorly understood. A key to understanding the exact features of chromatin that impinge on replication origin function is to define the precise locations of the DNA sequences that control origin function. In S. cerevisiae, Autonomously Replicating Sequences (ARSs) contain a consensus sequence (ACS) that binds the Origin Recognition Complex (ORC) and is essential for origin function. However, an ACS is not sufficient for origin function and the majority of ACS matches do not function as ORC binding sites, complicating the specific identification of these sites.
Results
To identify essential origin sequences genome-wide, we utilized a tiled …
引用总数
学术搜索中的文章
W Xu, JG Aparicio, OM Aparicio, S Tavaré - BMC genomics, 2006