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L. Jemeršić et al.: Hepatitis E virus persistence in pigs and wild boars in Croatia their geographical origin. Seropositive wild boars were found in six out of 16 counties, showing an overall seroprevalence of 31.10%(95% CI 28.31-34.04%), ranging from 7.70% to 50.60%. The highest seroprevalence was found in the Vukovar-Srijem and Osijek-Baranja Counties (North-East Croatia) where pig breeding dominates and the highest density of wild boars is recorded. While no positive heV rNa samples were detected in tested domestic pigs, seventeen serologically positive wild boars (11.33%; 95% CI 7.19-17.14) younger than one year of age were found to be heV rNa positive, indicating chronic infection and possible prolonged virus spread into the environment. the detected viral presence despite the immune response indicates that wild boars have a key role in heV epidemiology.