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Electromayogram signals are frequently used to evaluate muscle injuries. They are also used extensively in biofeedback training equipment. This study investigates the electrical activity of muscles and the interrelationship of muscles and nerve fibers. Muscle potentials are observed and measured on an oscilloscope. By placing electrodes in to a skeletal muscle we can monitor the electrical activity of the muscle. EMG is used to detect muscular disorder along with muscular abnormalities caused by other system disease such as nerve dysfunction. Neuro muscular disorder also can be known with the help of EMG. EMG is also used for diagnose of Neuro muscular performance. It is observed that there is alternating relationship between the contractions of biceps and triceps the relaxation of one forces the contraction of the other. EMG signals have been recorded at the various positions like point Biceps, point triceps and point contractions. The frequency range of the muscle spikes covers a bandwidth from DC to over 2 KHz. This recording belongs to a normal subject.