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Peak expiratory flow is the maximum rate of air expelled during forced expiration and is used clinically to monitor patients with asthma and other lung diseases. There are previous reports on the determinants of peak expiratory flow. Despite these reports, there is a dearth of knowledge on the actual role of postural alterations and gender-based variations in peak expiratory flow rate. Hence, this study sought to investigate the impact of postural changes on peak expiratory flow rate among young adults. Forty (40) healthy subjects between the ages of eighteen and twenty-four years (18-24 years old) were randomly selected for this study. The participants' peak expiratory flow rate (PEF), height, age, weight, and BMI (body mass index) were measured during the study. Data analysis was done using GraphPad Prism (version 5), data were presented as mean ± standard deviation and analyzed using a student t-test at p < 0.05. The mean weight and height of the male participants were higher when compared to the female participants (p < 0.05). The peak expiratory flow values in males in both sitting and standing postures were significantly higher when compared to the same postures in females. The study shows that the peak expiratory flow rate varies in different postures and among male and female gender in young adults. This will be useful in clinical practice and to monitor lung functions among young adults.