查看文章

Grape (V. vinifera L. cv Gamay Fréaux) cells were entrapped in calcium alginate beads. The effect of bead diameter and gel concentration on the viability of the cells was checked. The reduction of 2,3,5-triphenyltetrazolium chloride as well as O₂ consumption were used as viability test, and their results compared. The existence of diffusional limitations for O₂ introduces an unaccuracy as high as 25% for the O₂ consumption method. The reduction assay is more simple, precise and reliable.