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This research is aimed at finding the effects of: 1) organizational culture on performance, 2) leadership on performance; 3) achievement motivation on performance; 4) organizational culture on achievement motivation; 5) leadership on achievement motivation. This research used a survey method with a causal approach. There were 255 lecturers selected from initial population of 706 individuals based on a simple random technique as the sample. This research was conducted using questionnaires given to lecturers in Jambi University. The validity test was conducted using the Cronbach’s alpha. The results showed that: first, there was a direct effect of organizational culture on performance; second, there was a direct effect of leadership on performance; third, there was a direct effect of achievement motivation on performance; fourth, there was a direct effect of organizational culture on achievement motivation; fifth, there was a direct effect of leadership on achievement motivation. The implication of this research is that the effort to improve lecturer performance can be made by improving the organizational culture, leadership, and achievement motivation.