作者
Masaki Fujita, Kazuyoshi Kuwano, Ritsuko Kunitake, Naoki Hagimoto, Hiroyuki Miyazaki, Yumi Kaneko, Masayuki Kawasaki, Takashige Maeyama, Nobuyuki Hara
发表日期
1998/11/20
期刊
International archives of allergy and immunology
卷号
117
期号
3
页码范围
202-208
出版商
S. Karger AG
简介
Background: Studies have shown the importance of apoptosis in vascular injury in vitro. We postulated that apoptosis of the endothelium contributes to vascular injury in vivo and may be involved in acute lung injury. Methods: To test this hypothesis, we investigated the incidence of endothelial cell apoptosis in acute lung injury induced in mice by the administration of lipopolysaccharide (LPS). Male ICR mice were administered LPS (20 mg/kg body weight) intravenously and sacrificed at specified times thereafter. Results: Histologic findings were consistent with acute lung injury which increased with time from 3 to 48 h after injection. Electrophoretic analysis of DNA that was extracted from lung tissue and 3'–end–labeled with digoxigenin demonstrated a fragmentation of DNA starting at 6 h. In situ terminal deoxynucleotidyl transferase–mediated dUTP biotin nick end–labeling (TUNEL) demonstrated DNA …
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M Fujita, K Kuwano, R Kunitake, N Hagimoto… - International archives of allergy and immunology, 1998