作者
Alexander N Kapustin, John D Davies, Joanne L Reynolds, Rosamund McNair, Gregory T Jones, Anissa Sidibe, Leon J Schurgers, Jeremy N Skepper, Diane Proudfoot, Manuel Mayr, Catherine M Shanahan
发表日期
2011/6/24
期刊
Circulation research
卷号
109
期号
1
页码范围
e1-e12
出版商
Lippincott Williams & Wilkins
简介
Rationale:
Matrix vesicles (MVs) are specialized structures that initiate mineral nucleation during physiological skeletogenesis. Similar vesicular structures are deposited at sites of pathological vascular calcification, and studies in vitro have shown that elevated levels of extracellular calcium (Ca) can induce mineralization of vascular smooth muscle cell (VSMC)–derived MVs.
Objectives:
To determine the mechanisms that promote mineralization of VSMC-MVs in response to calcium stress.
Methods and Results:
Transmission electron microscopy showed that both nonmineralized and mineralized MVs were abundantly deposited in the extracellular matrix at sites of calcification. Using cultured human VSMCs, we showed that MV mineralization is calcium dependent and can be inhibited by BAPTA-AM. MVs released by VSMCs in response to extracellular calcium lacked the key mineralization inhibitor matrix Gla …
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AN Kapustin, JD Davies, JL Reynolds, R McNair… - Circulation research, 2011