作者
Alison Motley, Nicholas A Bright, Matthew NJ Seaman, Margaret S Robinson
发表日期
2003/9/1
期刊
The Journal of cell biology
卷号
162
期号
5
页码范围
909-918
出版商
Rockefeller University Press
简介
We have used RNA interference to knock down the AP-2 μ2 subunit and clathrin heavy chain to undetectable levels in HeLaM cells. Clathrin-coated pits associated with the plasma membrane were still present in the AP-2–depleted cells, but they were 12-fold less abundant than in control cells. No clathrin-coated pits or vesicles could be detected in the clathrin-depleted cells, and post-Golgi membrane compartments were swollen. Receptor-mediated endocytosis of transferrin was severely inhibited in both clathrin- and AP-2–depleted cells. Endocytosis of EGF, and of an LDL receptor chimera, were also inhibited in the clathrin-depleted cells; however, both were internalized as efficiently in the AP-2–depleted cells as in control cells. These results indicate that AP-2 is not essential for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of …
引用总数
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A Motley, NA Bright, MNJ Seaman, MS Robinson - The Journal of cell biology, 2003