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Fomitiporia punctata (P. Karst.) Murrill is a wood decay fungus on broadleaves. It has only recently been reevaluated as a distinct species with respect to Phellinus punctatus (P. Karst.) Pilát sensu lato species complex, a group taxonomically complex to discriminate. In turn, F. mediterranea M. Fisch. was recognized as an independent species in 2002 based on strains isolated from Vitis vinifera L. in Germany. Since that moment, the species has been increasingly reported in Europe and Southern regions particularly, with special focus on its pathogenicity for some cultivated woody plants. Nevertheless, it has not been clarified yet whether F. punctata and F. mediterranea are sympatric in south Europe and South of Alps in particular. Aim of this work was to investigate whether F. punctata and F. mediterranea are both present in Lombardia by considering different provinces and hosts. Samplings in different provinces and environments in Lombardia provided the culture collection of DSTAUnipv (MicUNIPV) with over 60 dikaryon strains in pure culture morphologically recognized as belonging to P. punctatus sensu lato. ITS analysis on a subset including strains from Pavia, Varese, Como and Brescia provinces revealed the presence of F. mediterranea only, whereas no strains were attributed to F. punctata. For a strain subset, LSU sequences were obtained as well. Interspecific discrimination was furtherly explored by both ITS and ITS-LSU approach with respect to all available sequences in Genbank belonging to P. punctatus sensu lato and including sequences specifically referred to F. mediterranea. The cladograms clearly show that all the …